Cell culturing is a key method used to isolate, analyze and grow human or animal cells under controlled conditions. While used in a variety of applications, cell culturing is heavily applied in biochemical, molecular, and physiological studies of metabolism and drug testing. Cell culturing helps understand how monoclonal antibodies, vaccines and therapeutic proteins interact with other chemicals for therapeutic studies.
Our cell culture inserts can be manufactured with a variety of Unique-Mem® track-etched membranes and are bonded directly to the insert. The combination of the membrane sealing process with the Unique-Mem® membrane creates a strong bond between the plastic body and a perfectly smooth membrane surface, usable even for large inserts and in unique larger cell culture formats like rectangular or round versions with growth areas of up to 100 cm2 or even larger.
Oxyphen track-etched membranes are a perfect fit for this application, given the highly controlled membrane performance parameters, the wide variability in membrane thickness and pore size to regulate the nutrient media transport to the cells that facilitates the correct growth. When combined with our membrane assembly processes, customers can choose from a variety of cell culture inserts based on either a standard hanging design for three well sizes (6-, 12- and 24-well inserts) or a customized design per customer-specific requirements.
For cell culturing, applications such as co-culture, secretion, cytotoxicity studies and drug transport research, smaller pore sizes are recommended (~ 0.4µm – 1.0µm). Pore sizes from 3.0µm up to 8.0µm are suggested for chemotactic studies and/or migration, invasion. The thicknesses of the membranes range from 8 µm to 23 µm. More specific technical information is found below.
- Excellent cell attachment and growth
- Best fluorescence imaging quality
- High visibility of particles and cells
- Backflushing allows reproducible sample recovery
- 100% Free of PFOA: Compliant with (EC) 1907/2006 REACH / Regulation (EU) 2019/1021 POP
|Rane Material||Pore Size||Application|
|PET||small 0.4 um||Co-Culturing
Drug Transport Studies
|PET||large 3.0 – 8.0um||Cell invasion
Transport of larger molecules (lipoproteins, viruses)
smooth muscle migration
|PC||TBD||Please talk to one our experts for PC as a material option.|
|Membrane Material||# of wells|
|PET||Multiwell plates (48 / 96 well)|
Multiwell plates (48 / 96 well)
Cell culture inserts (6 / 12 / 24 well)
|Air Flow||Up to 800 l / (bar cm2 min)|
|Pore Size||0.1µm to 10µm|
|Pore Density||0.01M to 1,000M pores per cm2|
|Thickness||8µm to 50µm|
|Temperature Range||– 40°C and 160°C (up to 200°C)|
|Hydrophilic Treatments||Various hydrophilic treatments increase water flow rate and wetting abilities of the membrane|
|Sterilization Procedures||Stable against Gamma and X-ray|
|Specification||Unique-Mem® Track-Etched Membranes||RoTrac® Track-Etched Membranes|
10, 13, 14.5, 20 & custom sizes
|High Quality Raw Materials||
PET – naturally slightly hydrophilic
PC – naturally slightly hydrophobic
PET-Membrane/ PET-Nonwoven –harder
PET-Membrane/ PP-Nonwoven – softer
|Pore Size Ranges||
0.1µm to 10µm
0.2µm to 3.0µm
|Pore Density Ranges||
105 to 109 pores per cm2
2×106 bis 3×108 pores per cm2
8µm to 50µm
90µm to 220µm
– 40°C and 160°C (for special application < 200°C)
– 40°C and 130°C (for special application < 160°C)
|Air Flow Ranges||
Up to 800 l / (bar cm2 min)
Up to 37 l / (bar cm2 min)
Various hydrophilic treatments increase water flow rate and wetting abilities of the membrane (example: PVP treatment)
|Hydrophobic / Oleophobic Treatment:||
Grade up to 7.5; AATCC TM 118
|Salt spray resistance test2||
According to IEC 60068-2-52, based on validation by the customer
|Temperature resistance test1||
In accordance with ISO 16750-4
|Ice water shock test1&2||
In accordance with ISO 16750-4
|Climate resistance test||
In accordance with ISO 16750-4
|Chemical resistance test1||
According LV124/ ISO 16750-5
IP 64/65/66/67/68, according to DIN 40050
1 Testing in Progress
2 Based on Customer Validation
Viral Filtration Efficiency (VFE) Certificate
Final test report from Nelson Labs for Viral Filtration Efficiency (VFE) at an Increased Challenge Level. Also available is the Bacterial Filtration Efficiency (BFE) report.
Bacterial Filtration Efficiency (BFE) Certificate
Final test report from Nelson Labs for Bacterial Filtration Efficiency (BFE) at an Increased Challenge Level. Also available is the Viral Filtration Efficiency (VFE) report.
OxyDisc® Product Brochure
Learn more about OxyDisc® die-cut membrane discs that can be produced using either hydrophobic or hydrophilic membranes and easily handled and mounted using multiple assembly options to suit a variety of industries.
Register for our On-Demand Webinar
Delivering Precision & Control: An Introduction to Track-Etched Membrane Technology
OxyPad® Self-Adhesive Pads
OxyPad® membrane pads, which are easily integrated inside or outside your housing design, promote consistent pressure equalization and protection against water, dust, and other harmful particulates.
OxyDisc® Die-Cut Membrane Discs
OxyDisc® die-cut membrane discs can be easily mounted and produced using either hydrophobic or hydrophilic membranes for a variety of filtration or venting application requirements.
OxySeal® Pressure Compensation Units
OxySeal® pressure compensated venting plugs, which are available with hydrophobic or oleophobic treated membranes, can prevent the ingress of liquid, water, dust, dirt, salt, and other contaminants.
Membrane Technologies Available
Unique-Mem® Unlaminated Track-Etched Membranes
Unique-Mem® track-etched membranes, available as hydrophilic or hydrophobic, are characterized by cylindrical shaped pores penetrating the membrane in different angles. They have a smooth flat surface and well-defined flow rates.
RoTrac® Laminated Track-Etched Membranes
RoTrac® track-etched membranes are Unique-Mem® membranes that are supported with non-wovens (PP or PET) to create a more robust membrane. They are available as either hydrophilic or hydrophobic membranes.
Interested in speaking with one of our track-etched membrane experts?